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wga hrp  (Vector Laboratories)


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    Vector Laboratories wga hrp
    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed <t>to</t> <t>WGA-HRP</t> served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).
    Wga Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 114 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/PL-1026-2/Peroxidase+conjugated+Wheat+Germ+Agglutinin+(WGA)/bio_rxiv__64898__2026__04__02__716088-313-72-73
    Average 93 stars, based on 114 article reviews
    wga hrp - by Bioz Stars, 2026-10
    93/100 stars

    Images

    1) Product Images from "GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis"

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis

    Journal: bioRxiv

    doi: 10.64898/2026.04.02.716088

    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).
    Figure Legend Snippet: (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Techniques Used: Negative Control, Membrane, Labeling

    Related Articles

    Transfection:

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis
    Article Snippet: RPE1 (centrin1-EGFP) cells were grown at a low density on gridded glass bottom dishes (MatTek). .. For depletion experiments, cells were transfected with siRNA during seeding as described above and serum-starved 48 h later for 4 or 12 h. When the Golgi apparatus was disrupted, cells were treated with 0.005% methanol or 0.5 μg/ml of BFA for 2 h in medium with serum before they were serum-deprived for 4 h. For each condition, cells were incubated for the last 15 min of serum starvation with 30 μg/ml of WGA-HRP (Vector Labs, cat. no. PL-1026-2) at 37°C. ..

    Incubation:

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis
    Article Snippet: RPE1 (centrin1-EGFP) cells were grown at a low density on gridded glass bottom dishes (MatTek). .. For depletion experiments, cells were transfected with siRNA during seeding as described above and serum-starved 48 h later for 4 or 12 h. When the Golgi apparatus was disrupted, cells were treated with 0.005% methanol or 0.5 μg/ml of BFA for 2 h in medium with serum before they were serum-deprived for 4 h. For each condition, cells were incubated for the last 15 min of serum starvation with 30 μg/ml of WGA-HRP (Vector Labs, cat. no. PL-1026-2) at 37°C. ..

    Article Title: Zebrafish gon4la mutants recapitulate human GON4L-related growth disorders and reveal novel metabolic organs abnormalities.
    Article Snippet: .. The sections were incubated overnight at 4°C with rhodamine-conjugated wheat germ agglutinin (WGA; 1:100 in buffer B, Vector Lab). ..

    Whole Genome Amplification:

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis
    Article Snippet: RPE1 (centrin1-EGFP) cells were grown at a low density on gridded glass bottom dishes (MatTek). .. For depletion experiments, cells were transfected with siRNA during seeding as described above and serum-starved 48 h later for 4 or 12 h. When the Golgi apparatus was disrupted, cells were treated with 0.005% methanol or 0.5 μg/ml of BFA for 2 h in medium with serum before they were serum-deprived for 4 h. For each condition, cells were incubated for the last 15 min of serum starvation with 30 μg/ml of WGA-HRP (Vector Labs, cat. no. PL-1026-2) at 37°C. ..

    Article Title: B lymphocytes contribute Angiotensin II induced cardiac hypertrophy
    Article Snippet: .. To assess cardiomyocyte size, paraffin sections were deparaffinized, rehydrated, and stained with rhodamine-conjugated wheat germ agglutinin (WGA; 5 μg/mL in 1% BSA, 1× TBS; Vector Laboratories). ..

    Article Title: NAMPT overexpression enhances the regenerative potential of mesenchymal stromal cell-derived extracellular vesicles in experimental AKI
    Article Snippet: Then, samples were incubated for 1 h at room temperature with a goat anti-rabbit Cy3-conjugated secondary antibody (1:100; Jackson ImmunoResearch Laboratories). .. Nuclei were stained with DAPI, and renal structures were labeled with FITC-conjugated wheat germ agglutinin (WGA) lectin (1:400; FL-1021 S, Vector Laboratories). ..

    Article Title: B lymphocytes contribute Angiotensin II induced cardiac hypertrophy.
    Article Snippet: .. To assess cardiomyocyte size, paraffin sections were deparaffinized, rehydrated, and stained with rhodamine-conjugated wheat germ agglutinin (WGA; 5 μg/mL in 1% BSA, 1× TBS; Vector Laboratories). ..

    Article Title: Zebrafish gon4la mutants recapitulate human GON4L-related growth disorders and reveal novel metabolic organs abnormalities.
    Article Snippet: .. The sections were incubated overnight at 4°C with rhodamine-conjugated wheat germ agglutinin (WGA; 1:100 in buffer B, Vector Lab). ..

    Article Title: Pregnancy hormones increase cardiac capillary density via the PGC-1α/ERRα/VEGF pathway in cardiomyocytes
    Article Snippet: The following antibodies were used for staining of fixated cells and heart sections (incubation for 2 h at RT in 0.2% Triton X-100 in PBS, 5% donkey serum or overnight at 4 °C in 0.2% Triton X-100 in PBS, 5% donkey serum): ASMAC (1:800, Sigma-Aldrich, Germany), pHH3 Ser 10 (1:100, Chemicon, Germany), Ki-67 (1:400, a gift from J. Gerdes, Forschungszentrum Borstel, Germany), ERRα (1:400, Santa Cruz Biotechnology, USA), PGC1-α (1:50, Santa Cruz Biotechnology, USA), and vimentin (1:800, Chemicon, Germany). .. Fluorescein Griffonia simplicifolia lectin (GSL, Vector Laboratories, USA) and wheat germ agglutinin (WGA, Vector Laboratories, USA) were diluted 1:100 and stained at RT for 1 h. Before Ki-67 staining, antigens were retrieved by boiling the sections in 10 mM sodium citrate buffer, pH 6.0, for 5 min in a microwave at 800 W. After washing, secondary antibodies conjugated to Cy2, Cy3, and Cy5 (1:400, Jackson ImmunoResearch, USA), diluted in 1 μg/mL Hoechst 33,342 (nuclear stain) in PBS, were applied for 1 h at room temperature. .. Sections were mounted with coverslips and imaged using an inverted fluorescence microscope (Axiovert 200M, Carl Zeiss, Germany) with filters for DAPI, GFP, Cy3, and Cy5; 25×, 40×, and differential interference contrast (DIC) plan apochromat oil objectives; an ebx 75 light source; and an AxioCam MRm digital camera and AxioVision Rel.

    Saline:

    Article Title: Functional inhibition of wheat germ agglutinin by glycodendrimers: Interplay of affinity, architecture, and temperature.
    Article Snippet: .. Horseradish peroxidase-conjugated wheat germ agglutinin (PL1026) was obtained from Vector Laboratories, Inc. Phosphate buffered saline (PBS) tablets, pH 7.4 a PBS tablet (T9181) was purchased from Takara Bio Inc. ELISA POD Substrate TMB Kit (Popular) (05298-80) was obtained from Nacalai tesque. ..

    Enzyme-linked Immunosorbent Assay:

    Article Title: Functional inhibition of wheat germ agglutinin by glycodendrimers: Interplay of affinity, architecture, and temperature.
    Article Snippet: .. Horseradish peroxidase-conjugated wheat germ agglutinin (PL1026) was obtained from Vector Laboratories, Inc. Phosphate buffered saline (PBS) tablets, pH 7.4 a PBS tablet (T9181) was purchased from Takara Bio Inc. ELISA POD Substrate TMB Kit (Popular) (05298-80) was obtained from Nacalai tesque. ..

    Staining:

    Article Title: B lymphocytes contribute Angiotensin II induced cardiac hypertrophy
    Article Snippet: .. To assess cardiomyocyte size, paraffin sections were deparaffinized, rehydrated, and stained with rhodamine-conjugated wheat germ agglutinin (WGA; 5 μg/mL in 1% BSA, 1× TBS; Vector Laboratories). ..

    Article Title: NAMPT overexpression enhances the regenerative potential of mesenchymal stromal cell-derived extracellular vesicles in experimental AKI
    Article Snippet: Then, samples were incubated for 1 h at room temperature with a goat anti-rabbit Cy3-conjugated secondary antibody (1:100; Jackson ImmunoResearch Laboratories). .. Nuclei were stained with DAPI, and renal structures were labeled with FITC-conjugated wheat germ agglutinin (WGA) lectin (1:400; FL-1021 S, Vector Laboratories). ..

    Article Title: B lymphocytes contribute Angiotensin II induced cardiac hypertrophy.
    Article Snippet: .. To assess cardiomyocyte size, paraffin sections were deparaffinized, rehydrated, and stained with rhodamine-conjugated wheat germ agglutinin (WGA; 5 μg/mL in 1% BSA, 1× TBS; Vector Laboratories). ..

    Article Title: Pregnancy hormones increase cardiac capillary density via the PGC-1α/ERRα/VEGF pathway in cardiomyocytes
    Article Snippet: The following antibodies were used for staining of fixated cells and heart sections (incubation for 2 h at RT in 0.2% Triton X-100 in PBS, 5% donkey serum or overnight at 4 °C in 0.2% Triton X-100 in PBS, 5% donkey serum): ASMAC (1:800, Sigma-Aldrich, Germany), pHH3 Ser 10 (1:100, Chemicon, Germany), Ki-67 (1:400, a gift from J. Gerdes, Forschungszentrum Borstel, Germany), ERRα (1:400, Santa Cruz Biotechnology, USA), PGC1-α (1:50, Santa Cruz Biotechnology, USA), and vimentin (1:800, Chemicon, Germany). .. Fluorescein Griffonia simplicifolia lectin (GSL, Vector Laboratories, USA) and wheat germ agglutinin (WGA, Vector Laboratories, USA) were diluted 1:100 and stained at RT for 1 h. Before Ki-67 staining, antigens were retrieved by boiling the sections in 10 mM sodium citrate buffer, pH 6.0, for 5 min in a microwave at 800 W. After washing, secondary antibodies conjugated to Cy2, Cy3, and Cy5 (1:400, Jackson ImmunoResearch, USA), diluted in 1 μg/mL Hoechst 33,342 (nuclear stain) in PBS, were applied for 1 h at room temperature. .. Sections were mounted with coverslips and imaged using an inverted fluorescence microscope (Axiovert 200M, Carl Zeiss, Germany) with filters for DAPI, GFP, Cy3, and Cy5; 25×, 40×, and differential interference contrast (DIC) plan apochromat oil objectives; an ebx 75 light source; and an AxioCam MRm digital camera and AxioVision Rel.

    Labeling:

    Article Title: NAMPT overexpression enhances the regenerative potential of mesenchymal stromal cell-derived extracellular vesicles in experimental AKI
    Article Snippet: Then, samples were incubated for 1 h at room temperature with a goat anti-rabbit Cy3-conjugated secondary antibody (1:100; Jackson ImmunoResearch Laboratories). .. Nuclei were stained with DAPI, and renal structures were labeled with FITC-conjugated wheat germ agglutinin (WGA) lectin (1:400; FL-1021 S, Vector Laboratories). ..

    other:

    Article Title:
    Article Snippet: Alexa Fluor-488-conjugated wheat germ agglutinin (WGA) , Vector Labs , Cat# FL-1021; N/A.



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    Image Search Results


    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Journal: bioRxiv

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis

    doi: 10.64898/2026.04.02.716088

    Figure Lengend Snippet: (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Article Snippet: For depletion experiments, cells were transfected with siRNA during seeding as described above and serum-starved 48 h later for 4 or 12 h. When the Golgi apparatus was disrupted, cells were treated with 0.005% methanol or 0.5 μg/ml of BFA for 2 h in medium with serum before they were serum-deprived for 4 h. For each condition, cells were incubated for the last 15 min of serum starvation with 30 μg/ml of WGA-HRP (Vector Labs, cat. no. PL-1026-2) at 37°C.

    Techniques: Negative Control, Membrane, Labeling