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wga hrp  (Vector Laboratories)


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    Vector Laboratories wga hrp
    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed <t>to</t> <t>WGA-HRP</t> served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).
    Wga Hrp, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 93/100, based on 111 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/PL-1026-2/bio_rxiv__64898__2026__04__02__716088-313-72-73?v=Vector+Laboratories
    Average 93 stars, based on 111 article reviews
    wga hrp - by Bioz Stars, 2026-08
    93/100 stars

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    1) Product Images from "GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis"

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis

    Journal: bioRxiv

    doi: 10.64898/2026.04.02.716088

    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).
    Figure Legend Snippet: (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Techniques Used: Negative Control, Membrane, Labeling



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    Image Search Results


    (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Journal: bioRxiv

    Article Title: GRAF1-dependent endocytotic processes and the Golgi apparatus contribute to novel intermediate stages of early ciliogenesis

    doi: 10.64898/2026.04.02.716088

    Figure Lengend Snippet: (a-f) Tomograms of RPE1 cells producing a centrin1-EGFP fusion protein. Cells were serum-starved for 4 hours and exposed to a fusion protein between wheat germ agglutinin (WGA) and horseradish peroxidase (HRP) for the last 15 minutes of serum starvation. (g, h) RPE1 cells which were not exposed to WGA-HRP served as a negative control. Red arrows point to the membrane of the nascent cilium, red arrow heads to tubular extensions. Shown in each case are three slices lying ∼46 nm apart (a), ∼55 and ∼80 nm nm apart (c), ∼55 nm apart (e), ∼55 and ∼110 nm apart (g). Respective cross sections of the nascent ciliary membrane structure are seen in b, d, f, h. (i) Quantification of nascent and mature cilia labeled with WGA-HRP in cells treated as described above. Over half of the nascent cilia and almost all the mature cilia were labeled by WGA-HRP. n , number of tomograms out of 2 independent experiments. Bars: 200 nm (a, c, e, g), 100 nm (b, d, f, h).

    Article Snippet: For depletion experiments, cells were transfected with siRNA during seeding as described above and serum-starved 48 h later for 4 or 12 h. When the Golgi apparatus was disrupted, cells were treated with 0.005% methanol or 0.5 μg/ml of BFA for 2 h in medium with serum before they were serum-deprived for 4 h. For each condition, cells were incubated for the last 15 min of serum starvation with 30 μg/ml of WGA-HRP (Vector Labs, cat. no. PL-1026-2) at 37°C.

    Techniques: Negative Control, Membrane, Labeling